We’re delighted to introduce Chrom Reaction Optimization (Chrom RO) 2.0. This release delivers a more compact, modern interface that preserves all functionality while improving clarity and flow. The result is a smoother experience throughout the analysis workflow. 

Improved Quantification Options 

Chrom RO 2.0 enhances quantification of reaction components—including isomers—by letting you define and select different chromatogram types (UV/PDA, MS, and others) per target. You can also tailor peak-picking settings per chromatogram—including EIC, so each trace is processed under conditions that best fit its signal characteristics. This leads to more reliable matching and more accurate quantitation across varied analytical scenarios.

Figure 1: New Chrom Reaction Optimization Settings dialog

Revamped Input Tab   

Choose how you search and how you quantify with updates to the Chrom RO Settings dialog: 

• New “Chromatogram” column: Select a preferred trace for quantification that can differ from the one used for target matching. 
• Condensed MS input: Fields previously spread across molfile, SMILES, molecular formula, monoisotopic mass, and m/z are now unified in a single Target MS column for faster setup. (See Figure 1)
• Dedicated, combinable dialogs: MS and RT (retention time) configuration dialogs are streamlined and separate, so you can mix and match constraints to get the results you want. 

Figure 2: Target MS and Target Retention Time dialogs

Enhanced LC/MS Evaluation Tab 

Build exactly the set of traces you need: 

• Editable chromatogram table: Add, remove, and edit chromatograms directly, including the Name column. 
• Work with multiple chromatogram types: Use UV/PDA, MS (including specific MS functions ±), and other modalities—each with its own settings for peak picking, alignment options, and more. 
• Per-trace peak picking: Configure peak-picking settings independently for every selected chromatogram, as well as for EICs generated during processing, for improved detection accuracy and overall results. 

Figure 3: New LC/MS Evaluation Tab 

Why Chrom RO 2.0 Matters 

Chrom RO 2.0 gives you fine-grained control over how targets are detected and quantified. By separating where you search from where you quantify, supporting MS-function selection, and enabling per-trace peak picking (including auxiliary EICs), you gain more flexibility to shape your analysis—and a faster route from raw data to confident decisions. 

Learn more: Watch this webinar to see Chrom RO 2.0 in action.